Basic Flow Cytometry Resources
- What is Flow Cytometry?
- Intro to Flow Cytometry
- How does a Flow Cytometer Work?
- Bio-Rad Flow Cytometry Basics Guide
- Cluster of Differentiation
- Spectral Cytometry
- Flow Cytometry Learning Center - Thermofisher
Online Tools and Assistance
- Compensation Tutorial
- Invitrogen Tutorials
- Chromocyte Training Resources
- Invitrogen Fluorescence Spectra Viewer
- BD Biosciences Spectra Viewer
- Biolegend Spectra Analyzer
- Cytek Spectra Viewer
- Invitrogen Tutorials
- Biolegend Multicolor Panel Selector
- Flow Repository
- Fluorofinder
- Cytobank
Instrument/Software Specific:
- DiVa 8.0 Basic Experimental Setup
- DiVa Compensation
- SpectroFlo Basic Expeerimental Setup
- SpectroFlow Plate Loader Setup
- Aurora Shutdown
- OpenFlow DiVa Experimental Setup
Flow Website Resources
- Purdue
- Purdue - Cytometry Mailing List
- CRUK FACS
- Mario Roederer - Compensation Tutorial
- Current Protocols in Flow Cytometry
- Human CD Marker Density
- huCD Marker Map
- International Society for Advancement of Cytometry
- Certification in Cytometry - SCYM (ASCP)
Commercial Training Resources
Please see the following links for some highly recommended commercial courses (Paid):
- Cheeky Scientist Cytometry Master Class
- Annual Flow Cytometry Course
- EMBL Courses
- BD Training
- MACS Academy
Important Flow Papers
- Seventeen-colour flow cytometry: unravelling the immune system.
- Interpreting flow cytometry data: a guide for the perplexed.
- A practical approach to multicolor flow cytometry for immunophenotyping.
- Quantum dot semiconductor nanocrystals for immunophenotyping by polychromatic flow cytometry.
- A new "Logicle" display method avoids deceptive effects of logarithmic scaling for low signals and compensated data.
- Amine reactive dyes: an effective tool to discriminate live and dead cells in polychromatic flow cytometry.
- Spectral compensation for flow cytometry: visualization artifacts, limitations, and caveats.
- Selecting fluorochrome conjugates for maximum sensitivity.
- Fluorescence spectral overlap compensation for any number of flow cytometry parameters.
- Flow cytometry controls, instrument setup, and the determination of positivity.
- Quality assurance for polychromatic flow cytometry using a suite of calibration beads
- Advanced analysis of human T cell subsets on the CytoFLEX flow cytometer using a 13 color tube based on DuraClone dry reagent technology
- Cell Sorting Guidelines
- Spectral Compensation for Linear-Logarithmic Flow Cytometry Acquisitions
Free Flow Analysis Software
- FCS Rename - edit FCS files!
- Web App for Flow Cytometry Analysis
BD Tech Notes
- Cell Sorting and Cell Preparation Tips
- Selecting Multi-colour Reagents
- PMT Optimization
- Fluorochrome Reference Chart
- Advantages of the 561-nm (Yellow-Green) Laser on the BD FACS Aria III
- BD Horizon Guided Panel Solution
- Compensation
Companies
Meet the Team
David Archer, PhD
Scientific Director, Pediatric+Winship Flow Cytometry Core and Animal Physiology Core
Aaron Rae
Core Technical Director, Pediatric+Winship Flow Cytometry Core
Igor Albizua-Santin
Flow Cytometry Technologist Supervisor
Ashley Jones, MS
Flow Cytometry Technologist, Sr