Sample Preparation
We strongly reccommend that you filter your cells prior to running on any of our instruments, there are various filter options available, please discuss with a member of the core facility. This is a great article on ways to prevent clumping:
Flow Cytometry Protocols To Prevent Sample Clumping
Running correct controls are very important, you should come with at least the following:
- Biological Controls
- FMO Controls for multi-color experiments to set Negative gates
- Compensation Controls
- QC Controls such as beads to take into account instrument changes over time.
- Proper Panel Design will also ensure you produce excellent data!
The flow core strongly recommends that you use a Live/Dead marker in all of your panels.
Meet the Team
David Archer, PhD
Scientific Director, Pediatric+Winship Flow Cytometry Core and Animal Physiology Core
Aaron Rae
Core Technical Director, Pediatric+Winship Flow Cytometry Core
Igor Albizua-Santin
Flow Cytometry Technologist Supervisor
Ashley Jones, MS
Flow Cytometry Technologist, Sr